EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) from EpiGentek

Product Specs
ItemEpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
CompanyEpiGentek
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP-9005
Quantity48 Assays, 96 Assays
ApplicationsAmount Quantitation
FormatMicroplate-based
Sample TypeRNA
Research AreaRNA Methylation

EpiGentek

110 Bi County Blvd. Ste 122

Farmingdale, NY 11735

United States

Phone: 1 877 EPIGENTEK (374-4368)
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(1) RNA N6-methyladenosine-modified-binding protein YTHDF1 promotes prostate cancer progression by regulating androgen function-related gene TRIM68Eur J Med ResDecember 2, 2023Qihong Nie, Xiaoyuan Wu, Yongming Huang, Tao Guo, Jin Kuang, Chuance Duquantification The m6A modification was detected using the EpiQuik m6A RNA Methylation Quantification Kit (P-9005; Epigentek Group Inc., USA). Briefly, 2 µL of negative control and 200 ng of RNA were added into strip

(2) N6-methyladenosine of TRIM27 enhances the stem cell-type phenotype of cisplatin-resistant colorectal cancer cellsBiochemistry and Biophysics ReportsDecember 1, 2023Jun-qiong Zheng, Ying Zhan, Wen-jing Huang, Zhi-yong Chen, Wei-hao Wupresence changes were quantified via EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) (#P-9005, Epigentek, Farmingdale, NY) in accordance with established guidelines provided by the manufacturer.

(3) METTL3 alters capping enzyme expression and its activity on ribosomal proteinsbioRxivNovember 26, 2023Daniel del Valle-Morales, Giulia Romano, Patricia Le, Michela Saviana, Rachel Brown, Lavender Micalo, Howard Li, Alessandro La La Ferlita, Giovanni Nigita, Patrick Nana-Sinkam, et al.levels were assayed using EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) (EpigenTek # P-9005-48) following manufacture’s protocol for absolute m6A levels with 200ng of total RNA. m6A RNA immunoprecipitation 7.5ug

(4) KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPNHeliyonNovember 8, 2023Yu Meng, Wenwen Yang, Jinchao Li, Wei Chaithe total RNA was determined using an EpiQuik m6A RNA Methylation Quantification Kit (colorimetric; P-9005, Epigentek, USA) according to the manufacturer's instructions and the m6A level was colorimetrically

(5) YTHDF1 mitigates acute kidney injury via safeguarding m6A-methylated mRNAs in stress granules of renal tubulesRedox BiologyNovember 1, 2023Wenwen Yang, Mingchao Zhang, Jiacheng Li, Shuang Qu, Fenglian Zhou, Minghui Liu, Limin Li, Zhihong Liu, Ke Zen1.44p. 2.7 m6A RNA methylation assay The amount of m6A RNA was detected by EpiQuik kit (Epigentek, P-9005). 200ug of total RNA was bound using a highly effective RNA binding solution. RNA with m6A modification

(6) Comprehensive Analysis of the Transcriptome-Wide m6A Methylome in Shaziling Pig Testicular DevelopmentInternational Journal of Molecular SciencesSeptember 23, 2023Chujie Chen, Xiangwei Tang, Saina Yan, Anqi Yang, Jiaojiao Xiang, Yanhong Deng, Yulong Yin, Bin Chen, Jingjing Gumodification amount of total RNA was quantified using the EpiQuik m6A RNA Methylation Quantification Kit (P-9005; Epigentek Group Inc., Farmingdale, NY, USA), following the manufacturer’s instructions. In brief, 200

(7) FTO-targeted siRNA delivery by MSC-derived exosomes synergistically alleviates dopaminergic neuronal death in Parkinson's disease via m6A-dependent regulation of ATM mRNAJournal of Translational MedicineSeptember 22, 2023Yan Geng, Xinyi Long, Yuting Zhang, Yupeng Wang, Guoxing You, Wenjie Guo, Gaoming Zhuang, Yuanyuan Zhang, Xiao Cheng, Zhengqiang Yuan, et al.level in total RNA was detected by the EpiQuik™ m6A RNA Methylation Quantification Kit (Colorimetric) (P-9005, EpiGentek, USA), according to the manufacture’s instruction. Small interfering RNAs (siRNAs) and Plasmids pCMV3-Flag-FTO,

(8) Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancerClinical and Translational MedicineSeptember 12, 2023Xiaoqiang Zhang, Xuyu Chen, Fangze Qian, Yanhui Zhu, Gao He, Junzhe Yang, Xian Wu, Hongfei Zhang, Xiafei Yu, Xiaoan Liumodification EpiQuik m6A RNA Methylation Quantification Kit was used to measure the amounts of m6A in total RNA (P‐9005; Epigentek Group Inc., Farmingdale, NY, USA). 200 ng of RNA were added to the assay wells together with

(9) Effects of Gestational Arsenic Exposures on Placental and Fetal Development in Mice: The Role of Cyr61 m6AEnvironmental Health PerspectivesSeptember 8, 2023Ya-Ping Song, Jin-Wei Lv, Zhi-Cheng Zhang, Qing-Hua Qian, Yi-Jun Fan, Dao-Zhen Chen, Heng Zhang, Fei-Xiang Xu, Cheng Zhang, Yichao Huang, et al.methylase activity/inhibition assay kit (#P9019-96) and EpiQuik m6A methylation quantification kit (#P-9005-48) were purchased from Epigentek. NE-PER nuclear and cytoplasmic extraction reagents (#78835) were

(10) Inhibition of CDK1 Overcomes Oxaliplatin Resistance by Regulating ACSL4‐mediated Ferroptosis in Colorectal CancerAdvanced ScienceSeptember 1, 2023Kaixuan Zeng, Weihao Li, Yue Wang, Zifei Zhang, Linjie Zhang, Weili Zhang, Yue Xing, Chi Zhouquadrants. m6A Detection Total m6A levels were detected using the m6A RNA Methylation Quantification Kit (#P‐9005‐48; Epigentek, NY, USA) or by immunofluorescence staining with anti‐m6A antibody (RRID:AB_2 924 958).

(11) Poly(GR) interacts with key stress granule factors promoting its assembly into cytoplasmic inclusionsCell reportsAugust 29, 2023Park J, Wu Y, Shao W, Gendron TF, van der Spek SJF, Sultanakhmetov G, Basu A, Castellanos Otero P, Jones CJ, Jansen-West K, et al.Fluorescence",Sigma-Aldrich,Cat#DUO82049 EpiQuik m6A RNA methylation quantification Kit,EpigenTek,Cat#P-9005 Coomassie Plus (Bradford) Assay Kit,Thermo Fisher Scientific,Cat#23236 Pierce™ BCA Protein Assay Kit,Thermo Fisher Scientific

(12) Expression profiling of N6-methyladenosine-modified mRNA in PC12 cells in response to unconjugated bilirubinMolecular Biology ReportsAugust 1, 2023Jinfu Zhou, Sining Liao, Chenran Zhang, Jinying Luo, Guilin Li, Huangyuan Liof the bilirubin and control groups were measured using an m6A RNA methylation quantification kit (P-9005; EpiGentek, Farmingdale, NY, USA). Briefly, the RNA-binding reagent was mixed with the total RNA extracted

(13) Genetic and pharmacological inhibition of METTL3 alleviates renal fibrosis by reducing EVL m6A modification through an IGF2BP2-dependent mechanismClinical and translational medicineAugust 1, 2023Ni WJ, Zhou H, Lu H, Ma NN, Hou BB, Li W, Kong FX, Yu JT, Hou R, Jin J, et al.level of total m6A in each group was measured using the EpiQuik m6A RNA Methylation Quantification Kit (P‐9005, EpigenTek) according to the manufacturer's protocol. Briefly, 200 ng of RNA was added dropwise to the

(14) Erythroid-transdifferentiated myeloid cells promote portal vein tumor thrombus in hepatocellular carcinomaTheranosticsJuly 31, 2023Wei-Hang Zhu, Jie Chen, Run-Kai Huang, Yuan Zhang, Ze-Xuan Huang, Xiu-Qing Pang, Bo Hu, Yang Yang, Xing Lithe EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) (Epigentek, Farmingdale, NY, USA, P-9005-48) following the manufacturer's instructions. The m6A level was quantified by measuring the absorbance

(15) The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roleseLifeJuly 25, 2023Imke Ensinck, Alexander Maman, Waleed S Albihlal, Michelangelo Lassandro, Giulia Salzano, Theodora Sideri, Steven A Howell, Enrica Calvani, Harshil Patel, Guy Bushkin, et al.standards. For ELISA, we either used the EpiQuik m6A RNA methylation quantification kit from EpiGentek (P-9005) according to the manufacturer’s protocol or an m6A ELISA protocol previous described (Ensinck et al.,

(16) Enhancement of TKI sensitivity in lung adenocarcinoma through m6A-dependent translational repression of Wnt signaling by circ-FBXW7Molecular CancerJuly 1, 2023Kai Li, Zi-Yang Peng, Rui Wang, Xiang Li, Ning Du, Da-Peng Liu, Jia Zhang, Yun-Feng Zhang, Lei Ma, Ye Sun, et al.methylation status of cells detected using the m6A RNA Methylation Quantification Kit (Epigentek, Cat#P-9005–113) according to the manufacturer’s instructions. In brief, 200 ng of total RNA was used as an input

(17) Tumor-associated macrophages promote cisplatin resistance in ovarian cancer cells by enhancing WTAP-mediated N6-methyladenosine RNA methylation via the CXCL16/CXCR6 axisCancer Chemotherapy and PharmacologyJuly 1, 2023Lan Hong, Xiuzhen Wang, Lang Zheng, Shengtan Wang, Genhai Zhu(Invitrogen), the levels of m6A were examined using a EpiQuik™ m6A RNA methylation quantification kit (P-9005-48, Colorimetric, Epigentek, Farmingdale, NY, USA). Subsequently, the levels of m6A were quantified

(18) METTL3-mediated m6A modification of SIRT1 mRNA inhibits progression of endometriosis by cellular senescence enhancingJournal of translational medicineJune 23, 2023Wang X, Wang J, Zhao X, Wu H, Li J, Cheng Y, Guo Q, Cao X, Liang T, Sun L, et al.levels among total RNAs were evaluated using a m6A RNA Methylation Quantification Kit (Colorimetric) (P-9005, Epigentek, USA). Total RNA was extracted using TRIzol extraction reagent (Ambion, USA), strips were

(19) Comprehensive analysis of differences in N6-methyladenosine RNA methylomes in Helicobacter pylori infectionFrontiers in Cell and Developmental BiologyJune 7, 2023Huan Li, Jiahui Lin, Sha Cheng, Jingshu Chi, Ju Luo, Yu Tang, Wenfang Zhao, Yufeng Shu, Xiaoming Liu, Canxia XuNanoDrop1000 (Thermo Fisher, United States). The EpiQuik m6A Methylation Quantification Kit (Epigentek, P-9005-96, United States) was used to measure the global m6A enrichment of mRNA. 200 ng RNA was coated in assay

(20) M6A modification promotes blood-brain barrier breakdown during cerebral ischemia/reperfusion injury through increasing matrix metalloproteinase 3 expressionHeliyonJune 1, 2023Liang E, Xiao S, Zhao C, Zhang Y, Fu G.RNA The m6A level in total RNA was detected using a commercial m6A RNA methylation quantification kit (P-9005, EpiGentek, United States). Briefly, 200 ng of total RNA was added into each well, and the capture antibody

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